Introduction of a Streptococcus cremoris plasmid in Bacillus subtilis.
نویسندگان
چکیده
Streptococcus cremoris Wg2 plasmid pWV01 was introduced in Bacillus subtilis by protoplast transformation. The yield of pWV01 isolated from B. subtilis was low. pWV01 contains a unique site for the restriction endonuclease MboI.
منابع مشابه
Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging.
Green fluorescent protein (GFP) offers efficient ways of visualizing promoter activity and protein localization in vivo, and many different variants are currently available to study bacterial cell biology. Which of these variants is best suited for a certain bacterial strain, goal, or experimental condition is not clear. Here, we have designed and constructed two "superfolder" GFPs with codon a...
متن کاملTransfer of Plasmids between Bacillus subtilis and Streptococcus lactis.
The shuttle plasmid pGK12, as well as several Staphylococcus aureus plasmids, was introduced into Streptococcus lactis by intergeneric protoplast fusion with Bacillus subtilis. The S. aureus plasmids were stably inherited in S. lactis, and so they may possibly be used as cloning vectors in lactic streptococci.
متن کاملCloning and Enhanced Expression of an Extracellular Alkaline Protease from a Soil Isolate of Bacillus clausii in Bacillus subtilis
in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...
متن کاملتأثیر Bacillus subtilis به عنوان پروبیوتیک بر فاکتورهای هماتولوژی و بیوشیمیایی خون ماهی قزل‌آلای رنگین‌کمان (Oncorhynchus mykiss) به دنبال عفونت تجربی با Streptococcus iniae
در این تحقیق جهت کنترل Streptococcosis با عامل Streptococcus iniae در ماهی قزل‌آلای رنگین‌کمان از Bacillus subtilis استفاده گردید. به طوری که از 2 گروه شامل کنترل و تیمار و سه تکرار برای هر گروه استفاده شد. درگروه کنترل، پروبیوتیک تجویز نشد اما در گروه تیمار، Bacillus subtilis با دوز107 سلول به ازای هر گرم غذا تجویز گردید. در روز چهل و پنجم تزریق داخل صفاقی Streptococcus iniae با دوز107×2 سلول...
متن کاملEditing of the Bacillus subtilis Genome by the CRISPR-Cas9 System.
UNLABELLED The clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) systems are adaptive immune systems of bacteria. A type II CRISPR-Cas9 system from Streptococcus pyogenes has recently been developed into a genome engineering tool for prokaryotes and eukaryotes. Here, we present a single-plasmid system which allows efficient genome editing of Bacillus subtilis Th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 156 2 شماره
صفحات -
تاریخ انتشار 1983